JRC GMO-Amplicons | EURL GMFF

Record ID:	HG996477.1
Database:	nt	Go to original record
Description:	Musa acuminata subsp. malaccensis strain Doubled-haploid Pahang (DH-Pahang) genome assembly, chromosome: putative_chloro_scaff
Taxon:	Musa acuminata subsp. malaccensis
Original size:	593221 bp

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Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48599	49192	-	594 bp

Amplicon:

594 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cttattttga gaaaacaaag tttataaaaa
151   ctagaattta aaaggatagg tgcagagact caatggaagc tgttctaacg
201   aatggagttg actacgtttt gttggtagca ggaatccgtc tataaaaatt
251   acagaaaaga tggtcctata cctaatacgt atgtatacat actgccatat
301   caaacgatta atcatgaccc gaatctatta tattataatt atatataatt
351   ataatatgaa aaattcagag ttattatgaa tccatttttc aatggaagtt
401   gaaggaagaa tcgaatattc aattatgaaa tcattcattc cagagtttga
451   tagatctttt gaaaactgat taatcggacg agaataaaga gagagtccca
501   ttctacatgt caataccgac aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt taaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	594	575	2.68e-08

Match details