JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860555.1
Database:	nt	Go to original record
Description:	Dryas octopetala tRNA-Leu (trnL) gene, intron.
Taxon:	Dryas octopetala
Original size:	590 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaatgg attgagcctt ggtatggaaa
51    cctaccaagt gagaactttc aaattcagag aaaccctgga attaaaaatg
101   ggcaatcctg agccaaatcc tgttttatga aaacaaacaa gggtttcaga
151   aagcgcgaat aaaaagggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttggctgcat tgtgttagta aaggaatcct tacatcgaaa
251   cttcagaaag gatgaaggat aaacgtatat acatacgtat acgtactgaa
301   atactatctc caaatgatta atgacgaccc gaatctgtct ttttttatat
351   ttatatgaaa aataaaagaa ttgttgtgaa tcgattctaa attgaaaaaa
401   gaatcgaata ttcattgatc aaatcattca ctccaccata gtctgataga
451   tctttttaag aattgattaa tcggacgaga ataaagatag agtcccattc
501   tacatgtcaa tattgacaac aatgaaattt atagtaagag gaaaatccgt
551   cgactttaga aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details