JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860554.1
Database:	nt	Go to original record
Description:	Dryas integrifolia tRNA-Leu (trnL) gene, intron.
Taxon:	Dryas integrifolia
Original size:	591 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	591	+	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttatg aaaacaaaca agggtttcag
151   aaagcgcgaa taaaaaggga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgca ttgtgttagt aaaggaatcc ttacatcgaa
251   acttcagaaa ggatgaagga taaacgtata tacatacgta tacgtactga
301   aatactatct ccaaatgatt aatgacgacc cgaatctgtc tttttttata
351   tttatatgaa aaataaaaga attgttgtga atcgattcta aattgaaaaa
401   agaatcgaat attcattgat caaatcattc actccaccat agtctgatag
451   atctttttaa gaattgatta atcggacgag aataaagata gagtcccatt
501   ctacatgtca atattgacaa caatgaaatt tatagtaaga ggaaaatccg
551   tcgactttag aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details