JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860549.1
Database:	nt	Go to original record
Description:	Diapensia lapponica tRNA-Leu (trnL) gene, intron.
Taxon:	Diapensia lapponica
Original size:	588 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	588	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaaa
101   atgggcaatc ctgagccaaa tcctgttttt cgaaaacaaa gaaagattca
151   gaaagcgaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gattgcgttg gtagaggaat ccttccatcg aaaccgcgga
251   aatgatgaaa gagaagggtc tatacatacg cactgaaata ctctatcaaa
301   tgattaatga cgactcaaat ctctatcctt tttttctatt tctataaaag
351   aaagaaagaa aaatagcaaa attgctgtaa atcgattcca cagtaaataa
401   agaatggaat gttcattgat caaatcattt actccagagt ctgatagatc
451   ttttgacgag ctgattaatc ggacgagaat aaagatagag tcccattcta
501   catgtcaata gcgacaacaa tgaaatttat agtaatagga aaatccgtcg
551   actttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details