JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860547.1
Database:	nt	Go to original record
Description:	Deschampsia brevifolia tRNA-Leu (trnL) gene, intron.
Taxon:	Deschampsia brevifolia
Original size:	613 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	613	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagcaaatcc atgttttgag aaaacaaggg ggttctcgaa
151   ctagaatgca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttaattaa gttgtgttgt tagtggaatt ccttctcgag
251   aaagaagggc tttatacatc taataaacac gtatagatac tgacatagca
301   aacgattaat cacagaaccc atattataat ataggttctt tattcttttt
351   tagcatgaaa tttgaaatag aaatgattat gaaataaaaa attcagaatt
401   tttttagaat tattgtgaat ccattccaat cgaatattta gtaatcaaat
451   ccttcaattc aaagttttga ggtctttaaa aaagtgtatt aatcggacga
501   ggtcaaagag agagtcccat tctacatgtc aatactgcca ataatgaaat
551   ttgctgtaag cggaaaatcc gtcgcgcttt gtaagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details