JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860546.1
Database:	nt	Go to original record
Description:	Ranunculus lapponicus tRNA-Leu (trnL) gene, intron.
Taxon:	Ranunculus lapponicus
Original size:	569 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	569	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tcgtatggaa
51    acatactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgctttcag aaaacaaaaa agggggtcca
151   gaaagcaaga atagggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatagagtt ggctgcgttg atcgagaaat ccttctattt taattcccga
251   aaggatgaac ccatatacgt acaaatacgt aataaaatag gaagcgacct
301   aaatctttat tttttatgtt atatgttata tgcaaaaata gaagccttct
351   tgtgaatcga ttccaagttg aagtaagaat cgaatattca ttgatcaaat
401   cagttattct ctaacctggt agatctttta aagaactaac tagttggacg
451   agaataaaga tagagtccca ttatacatgt caatatcaat accgacaaaa
501   atgaaattta tagtaagagg aaaatccgtc gacttttaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details