JRC GMO-Amplicons | EURL GMFF

Record ID:	MW044609.1
Database:	nt	Go to original record
Description:	Hippuris vulgaris isolate A chloroplast, complete genome.
Taxon:	Hippuris vulgaris
Original size:	152763 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46738	47302	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtctt ctcaaaacaa aggttcagaa
151   agcgaaacaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgttggta gaggaatctt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatcg attgaatact atatcaaata attaatgatg
301   gcccgaatct gtatctgtat ttttttatat gaaaaatgga agaattggtg
351   tgaattgatt ccacattgaa gaaagaatcg aatattcatt catcaaacga
401   ttcactccat agtctgatag atctttcttt taaagaactt attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaatattg acaacaatgc
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details