JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860545.1
Database:	nt	Go to original record
Description:	Chamerion latifolium tRNA-Leu (trnL) gene, intron.
Taxon:	Chamaenerion latifolium
Original size:	606 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	606	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgacaacttt caaattcaga gaaacccggg aattaaaaat
101   gggcaatcct gagccaaatc ctattttacg aaaaccaaca ccgggggggt
151   tagaaagcga gaaaaaagaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgatc gcgttgttgg taaaggcatt aatccttcta
251   tcaaaactcg agaaaggatg aaagagaaac ctatatacat atgtcgtgaa
301   tactctctca aataattaat gattttaaat gattaatgat ttgagattaa
351   tgacggcgcg aatttatttt ttttttttat gaaaaaagaa agaattgttt
401   gttgcgaatc aattctaagt tgaaggaaga atcgaatctt gattgagtaa
451   atcccttact ccacgaatct tttcaagaac ttattaatcg gatgagaata
501   aagagagagt cccattctac atgtcaatac cgacaataat gaaatttaga
551   gtaagaggaa aatccgtcga ctttaaaatc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details