JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860544.1
Database:	nt	Go to original record
Description:	Chamerion angustifolium tRNA-Leu (trnL) gene, intron.
Taxon:	Chamaenerion angustifolium
Original size:	605 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	605	+	605 bp

Amplicon:

605 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgacaacttt caaattcaga gaaacccggg aattaaaaat
101   gggcaatcct gagccaaatc ctattttacg aaaaccaaca ccggggggtt
151   agaaagcgat aaaaaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgatc gcgttgttgg taaaggaatt aatccttcta
251   tcaaaactcg agaaaggatg aaagagaaac ctatatacat atgtcgtgaa
301   tactctctca aataattaat gattttaaat gattaatgat ttgagattaa
351   tgacggcgcg aatcactttt tttttttatg aaaaaagaaa gaattgtttg
401   ttgcgaatca attctaagtt gaaggaagaa tcgaatcttg attgagtaaa
451   tcgcttactc cacgaatctt ttcaagaact tattaatcgg atgagaataa
501   agagagagtc ccattctaca tgtcaatacc gacaacaatg aaatttaaag
551   taagaggaaa atccgtcgac tttaaaatcg tgagggttca agtccctcta
601   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	605	586	2.73e-08

Match details