JRC GMO-Amplicons | EURL GMFF

Record ID:	MT671999.1
Database:	nt	Go to original record
Description:	Veronica ovata subsp. kiusiana chloroplast, complete genome.
Taxon:	Veronica ovata subsp. kiusiana
Original size:	152249 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46693	47266	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtctt ctaaaaaata aaaaaaaaaa
151   ggttcagaaa gtgaaaaaaa aaggataggt gcagagactc gatggaagct
201   gttctaacaa atggagttga ctgtgtcggt agatgaatct tttcatcgaa
251   acttcagaaa ggatgaagga taaagggatc tattgaatac tatataaaaa
301   taaaaaatta atggtgggcc gagtctgtat ctgtattttt tttagatgaa
351   aaatacaaga cttggtgtaa attgattcca cattcaagaa agaatggaat
401   attcatcaaa tcacccactc catagtctga tagatctttt aaagaactga
451   ttaatcgggc gagaataaag atagagtccc attctacatg tcaataccga
501   caaaaatgaa atttatagta agaggaaaat ccgtcgaatt taaaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details