JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860543.1
Database:	nt	Go to original record
Description:	Cerastium beeringianum tRNA-Leu (trnL) gene, intron.
Taxon:	Cerastium beeringianum
Original size:	590 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	590	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaatag gtttgagcct tagtatggaa
51    acctgctaag tgaaaacttt caaattcaga gaaaccctgg aaaaaaaagg
101   gcaatcctga gccaactcct taattgtctc ctaaaaaaaa cggattcaaa
151   aagaaagaat aaaaaaggat aggtgcagag actcaaagga agctgttcta
201   aaaaaaatgg ggacgtcctg aaatgattaa tgacaacccg aatttgttct
251   gtattttttt ttacttaatt atatatttat attataatta ataataatat
301   aatctctaga aatccatatt ataggataga tagatttaaa atatggaaaa
351   atgcaagaat tgttatggat cgattctaag ttgaaagccg aataaatctt
401   tgagttattc ataaaaacat tcatactaac cccatacata gtctgaaaga
451   tcttttgaag aagggattaa tcggatgaga ataaagatag agtcccgttc
501   tacatgtcaa tattgacaac aattaaattt atagtaagag gaaaatccgt
551   cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details