JRC GMO-Amplicons | EURL GMFF

Record ID:	MW362306.1
Database:	nt	Go to original record
Description:	Benincasa hispida chloroplast, complete genome.
Taxon:	Benincasa hispida
Original size:	156758 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49573	50165	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ctttttccga aaacaaaaaa aagggtaggt
151   gcagagactc aatggaagct gttctaacaa atggagttga ctacgttgcg
201   ttagtaaagg aatgaatcct tccatcgaaa ctccagaaaa gatgaaagat
251   aaacgtatta cgtactgaaa tactatattg attaatgaca actcgaatct
301   ctcttttttg atatttatat ataaatatca aaaatgatat atcaaaatga
351   acgaattgtt atgaatcgat tccaatatct ccaagttgaa aaaagaatcg
401   aatattcatt gatcaaatca tttactccat catacatagt ctgatagatc
451   ttttgaagaa ctgattaatc agatgcgaat agaataaaga tagagtccca
501   ttctacatgt caataccgac aaaaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details