JRC GMO-Amplicons | EURL GMFF

Record ID:	MW309817.1
Database:	nt	Go to original record
Description:	Alopecurus pratensis clone DKMN chloroplast, complete genome.
Taxon:	Alopecurus pratensis
Original size:	136157 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	102319	102938	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaagggggtt ctcgaactag
151   aatacaaagg aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatcgagtt aattatgttg tgttgttagt ggaattcctt ctaaattcga
251   gaaagaaggg ctttatacat ctaataaaca cgtatagata ctgacatagc
301   aaacgattaa tcacagaacc tatattataa ttataatata tggttcttta
351   ttctttttta gaatgaaata gaaatgatta tgaaataaaa aattcataat
401   ttttttttag aattattgtg aatacattcc aatcgaatat ttagtaatca
451   aatgcttcaa ttcaaagttt tgaggtcttt aatttaaaaa agtggattaa
501   tcggacgagg acaaagagag agtcccattc tacatgtcaa tactgacaac
551   aatgaaattt ctagtaaaag gaaaatccgt cgactttata agtcgtgagg
601   gttcaagtcc ctctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details