JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860542.1
Database:	nt	Go to original record
Description:	Castilleja hyperborea tRNA-Leu (trnL) gene, intron.
Taxon:	Castilleja hyperborea
Original size:	556 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	556	+	556 bp

Amplicon:

556 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaagaaa
101   aaagggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaata
201   aatggagttg attgcgctga tagaggaatg aaacttcaga aaggatgaag
251   gagaaacgta tctattgaat aatatatcaa atgatttatt aatgatggcc
301   caaatctgta tctgtatttt tgaatacgaa aagtggaaga attggtgtga
351   attccatatt gacgaaagaa tattcattca tcaaatcatt cactccatac
401   atagtctgat agatcctttt taaaagatct tattaatcgg acgagaataa
451   agatagagtc ccattctaca tgtcaatacc ggcaacaatg aaatttatag
501   taaaaggaaa atccgtcgac tttaaaaatc gtgagggttc aagtccctct
551   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	556	537	2.50e-08

Match details