JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860541.1
Database:	nt	Go to original record
Description:	Castilleja caudata tRNA-Leu (trnL) gene, intron.
Taxon:	Castilleja caudata
Original size:	563 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	563	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tagtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaagggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   taaatggagt tgattgcgct gatagaggaa tgaaacttca gaaaggatga
251   aggagaaacg tatctattga atactatata aaatgattta ttaatgatgg
301   cccaaatctg tatctgtatt tttgaatacg aaaagtggaa gaattggtgt
351   gaattccata ttgacgaaag aatattcatt catcaaatca ttcactccat
401   acatagtctg atagatcctt tttaatttaa aagatctgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caataccggc aacaatgaaa
501   tttatagtaa aaggaaaatc cgtcgacttt taaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details