JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860532.1
Database:	nt	Go to original record
Description:	Bupleurum americanum tRNA-Leu (trnL) gene, intron.
Taxon:	Bupleurum americanum
Original size:	583 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	583	+	583 bp

Amplicon:

583 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctttatt taatatttaa tttacaaaaa
151   caaacaaagg cccagaaggt gaaaaaggat aggtgcagag actcaatgga
201   agctgttcta acaactggaa ttgactgtgt tgcattggta gaggaatcct
251   tccattgaaa cttccgaaaa gatgaaggat gtacgtatat acatacgtac
301   tgaaatactc tatcaaatga ttaatgacga cctgcatctg tattttttat
351   atgaaaaacg gaaaaattgt tgggaatcga ttccatattg aagaaagaat
401   cgaatatgca ttgatcaaag catttacctc acggtctgat agttcttttg
451   tataactaat taatcggacg agaataaaga tagagtccta ttctacatgt
501   cattaccggc aacaatgaaa tttatagtaa gaagaaaatc cgttgatttt
551   aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	583	564	2.63e-08

Match details