JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860531.1
Database:	nt	Go to original record
Description:	Boykinia richardsonii tRNA-Leu (trnL) gene, intron.
Taxon:	Boykinia richardsonii
Original size:	579 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	579	+	579 bp

Amplicon:

579 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttacg aaaacaaaag acaagggttc
151   agaaagagag aatcaaaaaa aggataggtg cagagactcg atggaagcta
201   ttctaacaaa gggagtttat gttgcgttgg taaaggaatc cttctgtcga
251   aacttcagaa aggctgaagg ataaacctct atatatatgt atacgtactg
301   aaataatata tcaaatgatt aatgacgact cgaatctgta tttttttttt
351   ttataaaaag aattgttgta aatcgattcc aagttgaaga aagaatcgaa
401   tattcattga taaaaccatt cactccatag tctgataaat cctttgaaga
451   gctgattaat cagacgagaa taaagataga gtcccgttcg acatgtcaat
501   gcagacagca atgaaattta tagtaagagg aaaatccgtc gactttagaa
551   atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	579	560	2.61e-08

Match details