JRC GMO-Amplicons | EURL GMFF

Record ID:	KC439478.1
Database:	nt	Go to original record
Description:	Dracaena cambodiana tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Dracaena cambodiana
Original size:	1101 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	613	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aacgaaaaat
101   gggcaatcct gagccaaatc tttattttta gaaaaacaag ggttttcctt
151   ttattttaaa actagaagaa aaagggatag gtgcagagac tcaatggaag
201   ctgttctaac gaatggagtt gactacgttg cgttggtaac tagaatcctt
251   ctaaattaaa agataaatta aggaaaggat gacctatata tctaatacgt
301   acgtatacat actagcagat taatcacgac ctgaattcat tataattata
351   attataatga atttgcaata tatgcaaaat tcaaagttat tgtggatcta
401   tgccaatcaa agttgaagga aaaatcgaat attcagtgat caaatcgttc
451   attccagagt ttgataggcc tttttttttg aaaaattgat taatcggacg
501   agaataaaga gagagtccca ttctacatgt caataccgac aacaaggaaa
551   tttatagtaa gaggaaaatc cgtcgacttt agaaatcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details