JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860527.1
Database:	nt	Go to original record
Description:	Beckwithia chamissonis tRNA-Leu (trnL) gene, intron.
Taxon:	Ranunculus glacialis subsp. camissonis
Original size:	580 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	580	+	580 bp

Amplicon:

580 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acatactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgctttcag aaaacaaaaa ggggagttca
151   gaaagcaaaa atagggatag gtgcagagac tcaatggaag ctgttctaac
201   gaatagagtt gactgcgttg atcgaggaat ccttctattt gaattcccga
251   aaggatgaac ccatatacgt acgtacaaat acgtaataaa ataggaaaga
301   ggcgacccaa atctttcttt tttatgttat atgcaaaaca aatagaagaa
351   ttcttgtgaa tcgattccaa gttgaagtaa gaatcgaata ttcaatattc
401   attgatcaaa tcagttattc tctaacctgg tagatttttt aaagaactga
451   ctggttggac gagaataaag atagagtccc attatacatg tcaatatcaa
501   taccgacaaa aatgaaattt atagtaagag gaaaatccgt cgacttttaa
551   aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.61e-08
primerB	100.0	20 bp	0	0	580	561	2.61e-08

Match details