JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860526.1
Database:	nt	Go to original record
Description:	Astragalus umbellatus tRNA-Leu (trnL) gene, intron.
Taxon:	Astragalus umbellatus
Original size:	615 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	615	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgaaaacttt caaattcaga gaaaccctgg aattcacaat
101   gggcaatcct gagccaaatc ctgctttccg aaaacaaaaa aataaaagtt
151   cagaaagtta aaattaaaca aaaaaggata ggtgcagaga ctcaatggaa
201   gctgttctaa caaatggagt tgacaacatt tcctttcgca gtaggaaatg
251   aatccttcta tcaaaattcc agaaaggatc aaggataaac atatatattt
301   ttctatatat ttactgaaat actatttcag ttgattaatg aaaattacaa
351   acttatattt ggaaatattt cgattcgatt tcgatcacaa aagatgtcaa
401   tcaaatgaat tccaacttga agaaaaaatg taatattcat tgatcaaatc
451   agtcactcca acatagtctg atggatcttt tgaagaaaag attaatcaga
501   cgagaataaa gatagagtcc cattctacat gtcaataccg acaccaatga
551   aatttttagt aagaggaaaa tccgtcgact ttagaaatcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details