JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860525.1
Database:	nt	Go to original record
Description:	Astragalus polaris tRNA-Leu (trnL) gene, intron.
Taxon:	Astragalus polaris
Original size:	611 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	611	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgaaaacttt caaattcaga gaaaccctgg aattcacaat
101   gggcaatcct gagccaaatc ctgctttccg aaaacaaaaa aataaaagtt
151   cagaaagtta aaattggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt ggacaacatt aacatttcct ttcgcagtag gaaatgaatc
251   cttctataaa aattccagaa aggatcaagg ataaacatat atatgtttat
301   atatatttac tgaaatacta tttcagttga ttaatgaaaa ttacaaactt
351   atttttggaa atatttcgat tcgatttcga tcacaaaaga tgtgaatcaa
401   atgaattcca acttgaagaa aaaatgtaat attcattgat caaatcagtc
451   actccaacat agtctgatgg atcttttgaa gaaatgatta atcagacgag
501   aataaagata gagtcccatt ctacatgtca ataccgacac caatgaaatt
551   tttagtaaga ggaaaatccg tcgactttag aaatcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details