JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860520.1
Database:	nt	Go to original record
Description:	Armeria scabra tRNA-Leu (trnL) gene, intron.
Taxon:	Armeria scabra
Original size:	574 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	574	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattc gtttgagcct tagtatggaa
51    acctactaag tgacaacttt caaaatcaga gaaaccctgg aataaaaaaa
101   aatgggcaat cctgagccaa ctcccgcttt ccacataaaa aaggataggt
151   gcagagactc aatggaagct attctaacaa atgtcgttga cttcgttgca
201   ttgttataag aattcatcct ccaaaaagga tgaagaagaa acgtatatac
251   atatgtatac gtactgaaaa gtgaaacact atactgtatc gaaaaaaaga
301   attaatgatg acccgaatac atttttttca tattcataaa aaaaatggaa
351   gaattgttgt gaatttcttc caagttgaag aaagaatcta atatgaattt
401   atgcaattca aatgaaaaac ttaccccgtg tctgatagat cttttgaaga
451   accgatggat gagaatgaag atagagtccc attctacatg tcaataccga
501   caagaatgaa atttatagta agaggaaaat ccgtcgactt tataaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details