JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860519.1
Database:	nt	Go to original record
Description:	Arctophila fulva tRNA-Leu (trnL) gene, intron.
Taxon:	Arctophila fulva
Original size:	613 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	613	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaagaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tccttctaaa
251   ttcgagaaag aagggcttta tacatctaat aaacacgtat agatactgnc
301   atagcaaacg attaatcaca gaacctatat tataatatat ggttctttat
351   tcttttttag aatgaaatag aaatgattat gaaataaaaa attcagaatt
401   ttttttagaa ttattgtgaa tacattccaa tcgaatattt agtaatcaaa
451   tccttcaatt caaagttttg aggtctttaa aaaagtggat taatcggacg
501   aggacaaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details