JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860515.1
Database:	nt	Go to original record
Description:	Andromeda polifolia tRNA-Leu (trnL) gene, intron.
Taxon:	Andromeda polifolia
Original size:	566 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	566	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aaaaaaaaat
101   gggtaatcct gagccaaatc cttttttttt ggcaaacaaa caaagattcc
151   gaaagaaaaa aggataggtg cagagactca atggaagatg ttctaacaaa
201   tggagttaac tgcgttggta gaaagaatag gaattctttc atcgaaactt
251   tcgaaaagat gaaagataac tctatataca tacgcatagg taatgattaa
301   taacggccaa aatctgtagc cctatttttt tatatgaaaa atagacaaat
351   ttttgtgaat tgatttcaca ttgaataaag attggcatat ttattaatca
401   aatcatttac tccatcatct gctagttctt ttgaagaact gatgaatcgc
451   acgagaataa agatagagtc ccattctaca tgtcaatacc gacaagaatg
501   aaatttcttg taagaggaaa atccgtcgac ttttaaatcc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details