JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ860513.1
Database:	nt	Go to original record
Description:	Alnus viridis tRNA-Leu (trnL) gene, intron.
Taxon:	Alnus alnobetula
Original size:	607 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	607	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaata aaacaaattg
151   aagggttcat aaagcgagac taaaaaggat aggtgcagag actcaatgga
201   agctgttcta acaattgggg ttgacttcgt tccgttatta aagtaatcct
251   tctatcaaaa ctacagaaag gatgaaggat aaccgtatat acatatgtat
301   acgtactgaa atactatctc aaaaaattaa tgacgacctg aatctttatt
351   tctttttttt atatttatat aaaaactgaa tgaaagagtt gttgtgaatc
401   gatccaaatt gaagaaagaa tcaaatattt attaatcaaa ttatttactc
451   catcatagtc tgatagatct tttgaagaac taattaatcg gacgagaata
501   aagatagagt cccattctac atgtcaatac cgacaacaat gaaatttata
551   gtaagaggaa aatccgtcga ctttagaaat cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details