JRC GMO-Amplicons | EURL GMFF

Record ID:	AY881136.1
Database:	nt	Go to original record
Description:	Odontites serotina trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Odontites vernus subsp. serotinus
Original size:	1597 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	743	1285	+	543 bp

Amplicon:

543 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa agattcagaa
151   aacgaaaaaa ggagaggtgc agagactcta tggaagttgt tctaacaaat
201   ggagttgatt acgctggtag aggaatgaaa cttcagaaag gatgaaggca
251   tctattgact actatattaa tagattgatt aatgatggcc caaatctgta
301   tctgtatttt tgaatacgca aaatggaaga attggtgtga attgattcca
351   tattgaaaaa agaagattca ttgatcaaat aattcactcc atagtccgat
401   agatcgttta aagaactgat taatcggacg agaataaaga tagagtccca
451   ttctacatgt caataccggc aacaatgaaa tttatagtaa gaggaaaatc
501   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	543	524	2.44e-08

Match details