JRC GMO-Amplicons | EURL GMFF

Record ID:	AY881135.1
Database:	nt	Go to original record
Description:	Melampyrum roseum trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Melampyrum roseum
Original size:	1608 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	761	1313	+	553 bp

Amplicon:

553 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggca
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa ctcctgtttt ctcaaaacaa agattcagaa
151   aacaaaaaag gataggtgca gagactctat ggaagttgtt ctaacaaatg
201   gagttgatta cgctggtaga ggaatgaaac ttccgaaagg ataaaggcat
251   ctattgaata ctatatcaat atcaatatca ataaattgat taatgatggc
301   ccaatctgta tctgtatttt tgaatacgca aaagggaaga agtagtgtga
351   attgattcca tattgaaaaa agaagattca ttgatcaaat aattcactcc
401   ataatccgat agatcgttta aagaactgat taatcggacg agaataaaga
451   tagagtccca ttctacatgt caataccggc aacaatgaaa tttatagtaa
501   gaggaaaatc cgtcgacttt taaaatcgtg agggttcaag tccctctatc
551   ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	553	534	2.49e-08

Match details