JRC GMO-Amplicons | EURL GMFF

Record ID:	AY881134.1
Database:	nt	Go to original record
Description:	Euphrasia pectinata trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Euphrasia pectinata
Original size:	1602 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	758	1291	+	534 bp

Amplicon:

534 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaat
101   aatgggcaat cctgagccaa atcctgtttt ttccaaacaa agattcagaa
151   aacgaaaaaa aggataggtg cagagactct atggaagttg ttctaacaaa
201   tggagttgat tacgctggta gcgggatgaa actattgact actataatca
251   atagattgat taatgaaatg atggcccaga tctgtatctg tatttttttt
301   ttgaatacgc aaaatggaag aattggtgtg aattgattcc atattgaaaa
351   aagaagattc attgatcaaa taattcactc catagtccga tcgatcgttt
401   aaagaactga ttaatcggac gagaataaag atagagtccc attctacatg
451   tcaataccga caacaatgaa atttatagta agaggaaaat ccgtcgactt
501   ttaaaatcgt gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.40e-08
primerB	100.0	20 bp	0	0	534	515	2.40e-08

Match details