JRC GMO-Amplicons | EURL GMFF

Record ID:	AY881138.1
Database:	nt	Go to original record
Description:	Orobanche pycnostachya trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Orobanche pycnostachya
Original size:	1301 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	388	960	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acttgctaag tgataacttt caaattcaga gaaaccccgg aatccataaa
101   aagggccatc ctgagcccaa tcctatttta taaaaaaaaa aagtttataa
151   aatgaaaagg ggataggtgc agagactcaa tggaagttat tctaacaaac
201   agagttgatt gcgttggtag agaaatattt ccattgtaac ttcaaaaacc
251   cctgaaggat aaacatatct attaaatact atatactata taaactgatt
301   aatgataacc cgaacctata tatgtttttt tgaatattaa aaacagaaga
351   attgttgcga atttctttta tattgaagaa agaatattca ttcatcaaat
401   cattcactcc atagtctgat aaatttttta aaaaactgat taatcagacg
451   agaataaaga tagagtccca ttctacatgt caatacatgt caatactggc
501   aacaattaaa tttatagtaa gaggaaaatc cgtcgatttt aaaaatcatg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details