JRC GMO-Amplicons | EURL GMFF

Record ID:	AY881137.1
Database:	nt	Go to original record
Description:	Orobanche coerulescens trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Orobanche coerulescens
Original size:	1307 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	400	957	+	558 bp

Amplicon:

558 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tagtatggaa
51    acttgctaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctatttta tcaaaacaaa gtttagaaaa
151   cgaaaagtgg ataggtgcag agactcaatg gaagttgttc taacaaacag
201   agttgattgc gctggtagag aaatctttcc attgtaactt cagaaaggat
251   gaaggataaa cgtatctatt gaatactata taaactcatt aatatgaccc
301   gaacctanat ctgttttttg aatattaaaa agagaagaat tggtgtgaat
351   tttttatatt gaataaagaa tattcattaa taaatcattc actcaatagt
401   ctgataaatc ttttaaagaa ctgattaatc agacgagaat aaagatagag
451   tcccattcta caggtcaata caggtcaata ctggcaacaa ttaaatttat
501   agtaagagga aaatccgtcg attttaaaaa tcgtgagggt tcaagtccct
551   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	558	539	2.51e-08

Match details