JRC GMO-Amplicons | EURL GMFF

Record ID:	MW538953.1
Database:	nt	Go to original record
Description:	Clerodendrum bungei chloroplast, complete genome.
Taxon:	Clerodendrum bungei
Original size:	151678 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46657	47201	+	545 bp

Amplicon:

545 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttgctaag tgatgacttt caaattcaga gaaaccccgg aactaataaa
101   aatgggcaat cctgagccaa atcccgtttt ctcaaaacaa aggtttaaaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgctggta gaggcatttt tccatggaaa cttcagaaag
251   gatgaaggat aaatggatct attgaatact atatcgaatg attaatgatg
301   gctcgaatct gtatttttta atatgaaaaa tggaagaatt gattccacat
351   tgaagaaaaa atcgaatatt aattcatcaa atcattcact ccacagtccg
401   atagatcttt tcaagatctg attaatcgga cgagaataaa gatagagtcc
451   cgttctacat gtcaataccg gcaacaatga aatttatagt aagaggaaaa
501   tccgtcgact ttaaaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.45e-08
primerB	100.0	20 bp	0	0	545	526	2.45e-08

Match details