JRC GMO-Amplicons | EURL GMFF

Record ID:	MN556770.1
Database:	nt	Go to original record
Description:	Saxifraga prenja isolate MG16072937 tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, partial sequence.
Taxon:	Saxifraga prenja
Original size:	749 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	614	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttaag caagcaaaac acaagggttc
151   agaaaaagaa aaaaaaagga taggtgcaga gactcaatgg aagctattct
201   aacaaaggga gttgactgtg atgcggtggt aaaagaatcc ttccatcgaa
251   acttcagaaa ggcggaaggc taaactttta tacatattat ttattatgta
301   tacgtactga aatattatat aaaaataaaa tgataaattt aatattaatt
351   aatattaatg acgactcgag tctttttttt caacgaaaaa attgttgtaa
401   atcgattata agttgaagaa agaaaagaat tgaatattca ttgataaaac
451   cattcacact ctagtctgat aaatcctttg aagagctgat taatcagaca
501   agaataaaga tagagtccca ttctacatgt caatgctgac agcaatgaaa
551   tttttagtaa gaggaaaatc cgtcgacttt agaaatcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details