JRC GMO-Amplicons | EURL GMFF

Record ID:	MN556768.1
Database:	nt	Go to original record
Description:	Saxifraga paradoxa isolate MG16071611 tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, partial sequence.
Taxon:	Saxifraga paradoxa
Original size:	821 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3	609	+	607 bp

Amplicon:

607 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttaag caagcaaaac acaagggttc
151   aaaaaaagaa aaaaaaggat aggtgcagag actcaatgga agctattcta
201   acaaagggag ttgactgtga tgcggtggta aaagaatcct tccatcgaaa
251   cttcagaaag gctgaaggcg aaacttttat acatattatt tattatgtat
301   acgtactgaa atattttata aaaataaaat gataaattta atattaatta
351   atattaatga cgactcgagt cttttttttc aacgaaaaaa ttgttgtaaa
401   tcaattataa gttgaagaaa gaattgaata ttcattgata aaaccattca
451   cactctagtc tgataaatcc tttgaagagc tgattaatca gacaagaata
501   aagatagagt cccattctac atgtcaatgc tgacagcaat gaaattttta
551   gtaagaggaa aatccgtcga ctttagaaat cgtgagggtt caagtccctc
601   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	607	588	2.73e-08

Match details