JRC GMO-Amplicons | EURL GMFF

Record ID:	MN556756.1
Database:	nt	Go to original record
Description:	Saxifraga arachnoidea isolate MG16072311 tRNA-Leu (trnL-UAA) gene and trnL-trnF intergenic spacer, partial sequence.
Taxon:	Saxifraga arachnoidea
Original size:	823 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	613	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttaag caagcaaaac acaagggttc
151   agaaaaagaa aaaaaaggat aggtgcagag actcaatgga agctattcta
201   acaaagggag ttgactgtga tgcggtggta aaagaatcct tccatcgaaa
251   cttcagaaag gcggaaggct aaacttttat acatattatt tattatgtat
301   acgtactgaa atattatata aaaataaaat gataaattta atattaatta
351   atattaatga cgactcgagt ctttttttta aacgaaaaaa tttttgtaaa
401   tcgattataa gttgaagaaa gaaaagaatt gaatattcat tgataaaacc
451   attcacactc tagtctgata aatcctttga agagctgatt aatcagacaa
501   gaataaagat agagtcccat tctacatgtc aatgctgaca gcaatgaaat
551   ttttagtaag aggaaaatcc gtcgacttta gaaatcgtga gggttcaagt
601   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details