JRC GMO-Amplicons | EURL GMFF

Record ID:	MT447061.1
Database:	nt	Go to original record
Description:	Tabebuia nodosa plastid, partial genome.
Taxon:	Tabebuia nodosa
Original size:	158454 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47555	48125	+	571 bp

Amplicon:

571 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctttttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttggc tgcgttggta gaggaatctt tccatcgaaa cttcagaaag
251   gatgaaggat aaacgtatct attgaatact atatcaaatg attaatgatg
301   gcccgaatct gtatctgtat ttttttgtat ttttttatat gaaaaatgga
351   agaattagtg tgaattgatt ccacattgaa gaaagaatcg aatattcatt
401   catcaaatca ttcactccat agtccgatag ttcttttaaa gaactgatta
451   agcggacgag aataaagata gagtcccatt ctacatgtca ataccggcaa
501   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
551   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	571	552	2.57e-08

Match details