JRC GMO-Amplicons | EURL GMFF

Record ID:	JX235348.1
Database:	nt	Go to original record
Description:	Cryptochloa strictiflora chloroplast, partial genome.
Taxon:	Cryptochloa strictiflora
Original size:	135033 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47074	47686	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag cggtaacttc caaattcaga gaaacccagg aattaaaaaa
101   gggcaatcct gagccaaatc tgttttttga gaaaacaagc ggttctcgaa
151   ctagaatcca aaagaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttgattat gttgtgttgg cagtggaact ccctctaaat
251   tagagaaagt aagggcttta taaatctaat acacatgtat agatactgac
301   atagcaaacg attaatcaca gaacccatat cataatatag gttctttttt
351   cttttttaga atgaaattag gaatgattat gaaatacaaa attcttaatt
401   tttttaagat tcttgtgaat ctattccaat cgaatatgga gtaatcaaac
451   ccttcaattc acagttttcg agatctttta aaaaatagat taatcggacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details