Match details

Record ID: JX155046.1
Database: nt Go to original record
Description: Margaritopsis kappleri trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon: Eumachia kappleri
Original size: 1670 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 739 1328 + 590 bp

Amplicon: 590 bp
1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    actcgccgag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ctgaaagcaa aagttcagaa
151   agtgaaatta aaagggggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttggttgcgg tagtagagaa gtctttccat caaaaattca
251   gtaagtataa gtattaagga tggggtgaag gataaacgta tatacgtagt
301   gaatagtata ttaaacgaga aatgacagcc cgaatgaatt tatgtttttc
351   tatgaaaaaa caaagaattg gtttggtgtg aatagattct acgttgaaga
401   aagaatcgaa tattcattga tcaaatgatt gactccatag tctgataaat
451   cttttcaaga actgattaat tggatgagaa taaagataga gtcccattct
501   acatgtcaat gccggcagca atgaaattta tagttaagag gaaaatccgt
551   cgacttttaa aatcgtgagg gttcaagtcc ctctatcccc  

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.66e-08
primerB 100.0 20 bp 0 0 590 571 2.66e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.