JRC GMO-Amplicons | EURL GMFF

Record ID:	JX155042.1
Database:	nt	Go to original record
Description:	Chassalia sp. 2 VIET trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Chassalia sp. 2 VIET
Original size:	934 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	582	+	581 bp

Amplicon:

581 bp

1     cgaaatcggt agacgctacg gacttaatta gattgggcct tggtatggaa
51    actcgccgag tgagaacttt caaattcaga gaaaccccgg aattaataaa
101   agggggcaat cctgagccaa atcctgtttt ctgaaagaaa aagttcagaa
151   agttaaaaag gggataggtg cagagactca acggaagctg ttctaacaaa
201   tggagttggt tgcggtagta gagaagtctt tccatcaaaa attcagtaag
251   tataagtatt aaggatgagg tgaaggataa acgtatatac gtagtgaata
301   gtatattaaa cgagaaatga cagcccgaat gaatttatgt ttttctatga
351   aaaaacaaag aattggtgtg aatagattct acgttgaaga aagaatcgaa
401   tattcattga tcaaatgatt cactcccata gtcggataaa tcttttcaag
451   aactgattaa ttggatgaga ataaagatag agtcccattc tacatgtcaa
501   tgccggcagc aatgaaattt atagttaaga ggaaaatccg tcgactttta
551   aaatcgtgag ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.62e-08
primerB	100.0	20 bp	0	0	581	562	2.62e-08

Match details