JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102179.1
Database:	nt	Go to original record
Description:	Picrasma quassioides isolate P682 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Picrasma quassioides
Original size:	1059 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	603	+	593 bp

Amplicon:

593 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatcaaaaat
101   gggcaatcct gagccaaatc ctgttttaca agaacaagca agggttcaga
151   aagcgaaaaa ggggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga cttttggtaa agaaaagaaa atgaatcctt ctatcgaata
251   tcgaaacccc ataaaggaag aaggataagc gtatatacac tatgtataca
301   ctatctcaaa aatgacaacc gaatccttat ttctttttat gaaaaagaaa
351   agaagtgtta tgactcgatt ccaagttcaa gaaagaatcg aatattcatt
401   tatcaaatca ttcactccat agtctgatcg atcttttttt tctttttact
451   tttgaagaac tgattaatcg gaagagaata aagatagagt cccattctac
501   atgtcaatat caataccggc aacaatgaaa tttatagtaa gaggaaaatc
551   cgtcgacttt agaaatcgtg agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.67e-08
primerB	100.0	20 bp	0	0	593	574	2.67e-08

Match details