JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102178.1
Database:	nt	Go to original record
Description:	Sophora flavescens isolate P679 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Sophora flavescens
Original size:	1095 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	616	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttaccaag tgataacttt caaattcaga gaaaccctgg aattaataat
101   gggcaatcct gagccaaatc ctgtttttcg cgaaaccaaa gaaaagttca
151   taaagcgagc ataaagcgag aagaaaaagg ataggtgcag agactcaatg
201   gaagctgttc taacaaatgg agttgacgac atttcctttc gcattatatt
251   agggagggga tcaaagataa acatatatat ctatataata tacatatatg
301   gatatgtact gaaatattat ttcaattgat taatgaagac cgaaaatctc
351   tatttgtgaa tatttatatc acaaatgaaa agatgtgaag caaatcattt
401   ccaagttgaa gagaaagaat tcaatattta ttgatcaaat cattcattcc
451   atcacagtct gatagatctt ttgaagagct gattaatcag acgagaataa
501   agatagagtc ccattctaca tgtcaatatt gacaacaagt aaatttatag
551   taagaggaaa atccgtcgac tttagaaatc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details