JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102177.1
Database:	nt	Go to original record
Description:	Prunus persica isolate P678 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Prunus persica
Original size:	1058 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	600	+	590 bp

Amplicon:

590 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcgatcct gagccaaatc ctgttttatt aaaacaaaca agggtttcat
151   aaaccgagaa taaaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacaaatgga gttggctgca ttgtgttagt aaaggaatcc ttacatcgaa
251   acttccgaaa ggatgaagga taaacctata tgcatacgta tagtactgca
301   atagtatctc caaatgatta atgacggctc gaatctgtat ttttttatat
351   ttatatgaaa aacgaaagaa ttgttgtgaa tcaattaaaa attgaaaaaa
401   gaatcgaata ttcattgatc aaatcattca ctccatcata gtctgataga
451   tctttttaag aattgattaa tcggacgaga ataaagatag agtcccatta
501   tacatgtcaa tatcgacaac aatgaaattt atagtaagag gaaaatccgt
551   cgactttaga aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	590	571	2.66e-08

Match details