JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102176.1
Database:	nt	Go to original record
Description:	Rubus coreanus isolate P677 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Rubus coreanus
Original size:	1053 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	570	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ccgttttatg aaaacaaaca agggtttcag
151   aaagcgagaa taaataaagg ataggtgcag agactcaatg gaagctgttc
201   taataaatgg agttggcggc attgtgttcg taaaggaatc cttccatcga
251   aacttccgaa cggatgaaac ctatatacat atgtatactt actgaaatac
301   tatcgccaaa tgattaaaaa tgattaatga cgactccaac cggttctata
351   atttttatat atctatttag atgaaaaata gtcattgatc aaatcattca
401   ctccatcata gtctgataga tctttttaag aattgattaa tcggataaga
451   ataaagatag agtcccattc tacgtgtcaa tatcgacaac aatgaaattt
501   atagtaagag gaaaatccgt cgactttaga aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details