JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102174.1
Database:	nt	Go to original record
Description:	Sedum tosaense isolate P675 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Sedum tosaense
Original size:	937 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	596	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tcgtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaag
101   gggcaatcct gagccaaatc ttcttttccg aaaccaaatc aaaatagagg
151   ggtttaaaaa gtgagaataa aaaaggatag gtgcagagac tcaacggaag
201   ttattctaac aaatagagtt tactacattg cattggtaaa ggaatctttt
251   cagcgaaact ccagaaagga tgaagggtaa atcttaacat acatatggat
301   acgtaccgaa atagtatatc agatgattaa tgacgactca aatctttatt
351   taaaaatgaa agaattggta ggaagcgatt ccgaattgaa caaagaatct
401   attattcatt gataaaaaaa agtgtcactc cacagtttga tagatctttt
451   aacgaactga gattaatcgg acgagaataa agatagagtc ccattataca
501   cgtcaatact gacaacaagg aaattgatag taaaaggaaa atccgtcgat
551   tttagaaatc atgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details