JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102171.1
Database:	nt	Go to original record
Description:	Maclura tricuspidata isolate P395 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Maclura tricuspidata
Original size:	1050 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	585	+	575 bp

Amplicon:

575 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctaccaag tgacaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc cggttttctg aaaacaaaca agggttcaga
151   aggcaataat aaaaaaggat aggtgcagag actcaatgga agctgttcta
201   acaaatggag ttggctgcgg tgcgttagta aaggaatcac tccagaaaga
251   atgaagaata aacgtatata tgtatatata ctgaaatact atcttcaaat
301   gattaatgac aacccaaatc cgtatttctt ttcattttca tgaaaaatga
351   aagaattgtt gtgaatcaat tataaggtga aaaaagaatc aaatatttat
401   tgatcaaatc atttactcca tcaaaatctg atagatcttt tgaagaattg
451   attaatcgga cgagaataaa gatagagtcc tattctacat gtcaatatcg
501   gcaacaatga aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg
551   tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.59e-08
primerB	100.0	20 bp	0	0	575	556	2.59e-08

Match details