JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102170.1
Database:	nt	Go to original record
Description:	Cornus macrophylla isolate P394 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Cornus macrophylla
Original size:	1022 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	605	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tcgaaaacaa acaaaagttc
151   agaaagcgaa aataaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgact acgttgcgtt ttagaattag aagaatcttt
251   tcatcgaaac ttcagaaagg atgaaggata aaactatata catacgtata
301   cgtactgaaa tattatatca aatgattaat gacaacacga atctgtatct
351   gtagtttttt atatgaaaaa tcgaagaatt gttgtgaatc gattccaaat
401   tgaagaaaga atcaaatatt aattgatcaa atcatgcact ccatagtcgg
451   atagatcttt taaagaactg attaatcgaa cgagaataaa gatagagtcc
501   cattctacat gtcaataccg acaacaatga aatttatagt aagaggaaaa
551   tccgtcgatt ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details