JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102169.1
Database:	nt	Go to original record
Description:	Rhamnella franguloides isolate P393 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Rhamnella franguloides
Original size:	1068 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	605	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg ggcttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccctgg aattaaaacc
101   gggcaatcct gagccaaatc ctgttttctg aaaacaaaca aaagttcaga
151   aagcgataat aaaaaaggat agatagggat aggtgcagag actcaatgga
201   agttgttcta acaaatggag ttggccacga tgcgttagta aaggactcct
251   tccatcgaaa ctccagaaag tatgaagaat aaacgtatat atacgtactg
301   aaatactatc tccaaaccaa atgattaatg acgacccgaa tctttttttt
351   tttttttata taaaaaatga aagaattgtt gtgaatcgat tccaagttga
401   aaacagaatc gaatattcat taatcaaatc atttactcca tcgtaatctg
451   atagatcttt tgaagaattg attaatcgga cgagaataaa gatagagtcc
501   cgttctacat gtcaatatcg acaacaatgc aatttatagt aaaaggaaaa
551   tccgtcgact ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details