JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102167.1
Database:	nt	Go to original record
Description:	Trachelospermum asiaticum isolate P390 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Trachelospermum asiaticum
Original size:	1001 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	570	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag cgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctattttc cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggcgc agagactcga cggaagctgt tctaacaaat
201   ggagttggcc gcgttggtag agaaaccttt ccatcgaaaa ttcagaaagg
251   atggaggata tacctatgta ttgaatacta tatcaaatga ttaatgccga
301   cccgaatgaa tctatatttt tttctataaa aaacggaaga attggtgtga
351   ttcgattctt tcttcaatgt gggatcgaat attcattgat caaacgattc
401   actccatagt ccgtagatcc tcttttcaag aacggattaa tcggacgaga
451   ataaagatag agtcccgttc tacatgtcaa tgctggcaac aatgaaattt
501   atagtaagtg gaaaatccgt cgacttaaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details