JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102166.1
Database:	nt	Go to original record
Description:	Actinidia arguta isolate P388 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Actinidia arguta
Original size:	1032 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	598	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctttttt tcgaaaacaa acaaagattc
151   agaaagcgaa aataaaacaa ggataggtgc agagactcaa tggaagctgt
201   tctaataaat ggggttaact gcgtcggtag aggaatcctt ccatcgaaac
251   ttcagaaagg atgaaagaga aacctatata catacgcata cgtactgaaa
301   tacttaatca aatgattaat gacgactaaa atgggtatcc gtattttttt
351   tatgaaaaat ggacgaattg ttgtgaatcg attccccatt gaataaagaa
401   ttgaatattt atttattgat caaatcattt actccatagt ctgatagatc
451   tttttaagaa ctgattaatc ggacaagaat aaagatagag tcccattcta
501   catgtcaata ccgacaacaa tgaaattgat agtaagagga aaatccgtcg
551   actttagaaa tcgtgagggt tcaagtccct ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details