JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102165.1
Database:	nt	Go to original record
Description:	Asparagus schoberioides isolate P387 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Asparagus schoberioides
Original size:	1041 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	611	+	601 bp

Amplicon:

601 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tagtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc tttatgttta gaaaaacaag ggttttcatt
151   taaaaactag aagaaaaagg gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg gagttgacta tattgcgttg gtaaccggaa tccttctaaa
251   ttaaagaaag gatgacctat atatctaata cgtacgtata catactggca
301   tatcaaacga ttaatcacga cccgaatcca tattatatat aatatatgca
351   agacatgcaa aattcagagt tattatggat ctatgccaat agaagttgaa
401   ggaagaatcg aatattcagt gatcaaatga ttcattccag agtttgatat
451   accttttttt gaaaaattga ttaatgatta atcggacgag aataaagaga
501   gagtcccatt ctacatgtca ataccgacaa caatgaaatt tatagtaaaa
551   ggaaaatccg tcgactttag aaatcgtgag ggttcaagtc cctctatccc
601   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.71e-08
primerB	100.0	20 bp	0	0	601	582	2.71e-08

Match details