JRC GMO-Amplicons | EURL GMFF

Record ID:	JN102163.1
Database:	nt	Go to original record
Description:	Staphylea bumalda isolate P383 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Staphylea bumalda
Original size:	1042 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	605	+	595 bp

Amplicon:

595 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aatcaaaaat
101   gggcaatcct gagccaaatc ctggtttacg aaaacaaaca agaattcggc
151   aagcgagaat acaaaaagga taggtgcaga gactcaacgg aagcttttct
201   aacaaatgga attaactgcg atacgttgcg ttggtaaagg aatcctttta
251   gcgaaactcc agaaaggatg aaggataaac ctatatacat acgtatacgt
301   aatgacatac tatctcaaat gattaattta atgacgaccc gaatctgtat
351   tgtatttttt atatgaaaaa tgaaaaaatt gttgtgaatc gattccaagt
401   tgaaaaaaga atcaaatatt cattgatcaa ctcattcact ccgaagtctg
451   atagatcttt ttaagaactg attaatcaga cgagaataaa gatagagtcc
501   cattctacat gtcaataccg acaacaatga aatttagagt aagaggaaaa
551   tccgtcgact ttagaaatcg tgagggttca agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.68e-08
primerB	100.0	20 bp	0	0	595	576	2.68e-08

Match details